BBM

BBM Medium recipe

For 1 L Total


 		Component

Amount (ml/l)

Stock Solution (g/l dH2O)
1 NaNO3 10 25,00
2 CaCl2.2H2O 10 2.50
3 MgSO4.7H2O 10 7.50
4 K2HPO4 10 7.50
5 KH2PO4 10 17.50
6 NaCl 10 2.50
7 EDTA solution 1 see following recipe
8 Acidifed iron solution 1 see following recipe
9 H3BO3
 1 11.42
10
 Trace metals solution
 1
 see following recipe

EDTA solution


Component Amount (g/l dH2O)

1 EDTA 50.00
2 KOH 31.00

Acidified Iron Solution


 		Component Amount (to100 ml dH2O)

1 FeSO4.7H2O 0.498 g
2 H2SO4 (96%) 0.1 ml
 

Trace metals solution


Component Amount (g/l dH2O)

1 ZnSO4.7H2O 8.82
2 MnCl2.4H2O 1.44
3 MoO3 0.71
4 CuSO4.5H2O 1.57