Conway

Conwy Medium (Walne, 1974) Recipe

      For 1 L Total

Component

Amount
1 Sodium Nitrate 100 g
2 EDTA, disodium salt 45 g
3 Boric Acid 33.6 g
4 Sodium Phosphate, monobasic 20 g
5 Ferric Chloride, 6-hydrate 1.3 g
6 Manganous Chloride, 4-hydrate 0.36 g
7 Trace Metal Solution 1 ml
8 Vitamin Mix 100 ml
9 Distilled water (to make 1000 ml)

 

Trace Metal Stock Solution

 Component

Amount
1 Zinc Chloride 2.1 g
2 Cobalt Chloride, 6-hydrate 2.1 g
3 Ammonium Molybdate, 4-hydrate 2.1 g
4 Copper Sulfate, 2.0 g
5 Distilled Water 100 ml

It is necessary to acidify this solution with HCl by dropping the HCl until obtain clear liquid)

(The valid of this media should be not exceed 3 months period

 

Vitamin Mix

 Component

Amount
1 Vitamin B12 10.0 mg
2 Vitamin B1 20.0 mg
3 Distilled water 200 ml