F/2

F/2 Medium reciepe

For 1 L Total



 Component

Amount

(ml/l)

Stock Solution

Concentration (g/100ml dH2O)
1 NaNO3 1 7.5
2 NaH2PO4·H2O 1 0.5
3 Na2SiO3·9H2O 1 2
4 Trace Metals Solution 1
5 Vitamin B12 Solution 1
6 Biotin Vitamin Solution 1
7 Thiamine Vitamin Solution 1
 
 
Trace Metals Solution
 

 Component

Amount

(ml/l)

Stock Solution

Concentration (g/100ml dH2O)
1 ZnSO4•7H2O 23 0.08
2 MnSO4•H2O 152 0.9
3 Na2MoO4•2H2O 7.3 0.03
4 CoSO4•7H2O 14 0.05
5 CuCl2•2H2O 6.8 0.04
6 Fe(NH4)2(SO4)2•6H2O 4.6 g/l 11.7
7 Na2EDTA•2H2O 4.4 g/l 11.7

 

 Vitamin B12 Solution

 Component

Amount

(g/200 ml dH2O)
1 HEPES buffer pH 7.8 2.4
2 Vitamin B12 0.027

 

 Biotin Vitamin Solution

 Component

Amount

(g/200 ml dH2O)
1 HEPES buffer pH 7.8 2.4
2 Biotin 0.005

 

 Thiamine Vitamin Solution

 Component

Amount

(g/200 ml dH2O)
1 HEPES buffer pH 7.8 2.4
2 Thiamin 0.067