Mes Volvox

Mes Volvox Medium Recipe


 Component

Amount (ml/l)


 Stock Solution
Concentration (g/100 ml dH2O)
1 Ca(NO3)2·4H2O 1
 11.8
2 MgSO4·7H2O 1 4
3 Na2glycerophosphate.5H2O 1 5
4 KCl 1 5
5 MES 1.95 g/1
6 NH4Cl 1 2.67
7 P-IV Metal Solution 6 see following recipe
8 Vitamin B12 Solution 1 see following recipe
9 Biotin Vitamin Solution 1 see following recipe
10
 Distilled Water
 987 ml

P-IV Metal Solution


 Formula

Amount


1 Na2EDTA•2H2O 0.75 g/1
2 FeCl3·6H2O 0.097g/1
3 MnCl2·4H2O 0.041 g/1
4 ZnCl2 0.005 g/1
5 CoCl2·6H2O 0.002 g/1
6 Na2MoO4·2H2O 0.004 g/1

Vitamin B12 Solution


 Formula

Amount


1 HEPES buffer pH 7.8 2.4 g/200ml
2 Vitamin B12 0.027 g/200ml

Biotin Vitamin Solution


 Formula

Amount


1 HEPES buffer pH 7.8 2.4 g/200ml
2 Biotin 0.005 g/200ml