Modified Johnson
 
 Modified Johnsons Medium Recipe (Borowitzka, M.A., 1988).

Components Amount

1
NaCl
as needed to obtain desired salinity
2 MgCl2·6H2O 1.5 g
3 MgSO4·7H2O 0.5 g
4 KCl 0.2 g
5 CaCl2·2H2O 0.2 g
6 KNO3 1.0 g
7 NaHCO3 0.043 g
8 KH2PO4 0.035 g
9 Fe-solution 10 ml
10 Trace-element solution 10 ml
11
Distilled Water
980ml
 
 

Fe solution (for 1 liter)


Components Amount

1
Na2EDTA
189 mg
2
FeCl3·6H2O
244 mg
 
 

Trace-element solution (for 1 liter)


Components Amount

1 H3BO3 61.0 mg
2 (NH4)6Mo7O24·4H2O 38.0 mg
3 CuSO4·5H2O 6.0 mg
4 CoCl2·6H2O 5.1 mg
5 ZnCl2 4.1 mg
6 MnCl2·4H2O 4.1 mg

Adjust pH to 7.5 with HCl